PLK1 Antikörper (Biotin)

Produktnummer ABIN7658764

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PLK1 in WB und IP. Er zeigt eine Reaktivität gegenüber Xenopus laevis.

Kurzübersicht für PLK1 Antikörper (Biotin) (ABIN7658764)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reaktivität: Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PLK1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: AZ44

Produktdetails anti-PLK1 Antikörper (Biotin)

Verwendungszweck: PLK1 (Marker of Mitosis)(AZ44), Biotin conjugate

Produktmerkmale: Plk (for polo-like kinase) encodes a serine/threonine kinase that is closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. Polo and Cdc5 both code for proteins that are involved in regulating the function of the mitotic spindle. Plk protein accumulates in the cell during the S and G2 phases of the cell cycle, Plk protein content and catalytic activity peak at the onset of mitosis, followed by a rapid reduction after mitosis. Plk expression is detectable in mitotically active tissues such as colon and placenta, as well as in tumors of various origins. It has also been suggested that Plk may serve as a marker of cell proliferation. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. Useful for confirming that extracts have maintained CSF status and have not yet been activated into interphase. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length PLK1 Xenopus laevis protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Does not react with Human or Mouse, ELISA: For coating, order antibody without BSA, Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined by user.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Kommentare:

Positive Control: Mitotic CSF Xenopus egg extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Andere Bezeichnung: PLK1

Hintergrund:

Synonyms: Cell cycle regulated protein kinase, Polo like kinase 1 (PLK1), Serine/threonine-protein kinase PLK1, STPK13

Gene Symbol: plk1.S

Molekulargewicht: 66 kDa

Gen-ID: 380481

UniProt: P70032

Pathways: Zellzyklus, M Phase