FSHR Antikörper (CF®740)

Produktnummer ABIN7654181

Der Maus Monoklonal Anti-FSHR-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, FSHR in Proben von Human zu detektieren.

Kurzübersicht für FSHR Antikörper (CF®740) (ABIN7654181)

Target: FSHR (Follicle Stimulating Hormone Receptor (FSHR))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FSHR Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: FSHR-1400

Produktdetails anti-FSHR Antikörper (CF®740)

Verwendungszweck: FSH-Receptor (Ovarian Marker) (FSHR/1400), CF740 conjugate

Produktmerkmale: Follicle-stimulating hormone receptor (FSHR) is a 695 amino acid G protein coupled receptor. FSH binds to the receptor in a hand-clasp fashion via its alpha and beta subunits. While the beta subunit of FSH is involved in the binding of FSH to the receptor, the alpha subunit stabilizes this interaction. Linkage studies suggest that a missense mutation in the FSHR gene can cause reduced FSH binding affinity and lead to a condition known as hypergonadotropic ovarian dysgenesis (ODG). In males however, this mutation does not appear to have a detrimental affect on fertility. It is believed that a mutation in the FSHR gene is also associated with ovarian hyperstimulation syndrome, a condition characterized by the presence of multiple serous and hemorrhagic follicular cysts lined by luteinized cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length FSHR protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin) 1-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Uterine Carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu FSHR

Target: FSHR (Follicle Stimulating Hormone Receptor (FSHR))

Andere Bezeichnung: FSH Receptor

Hintergrund:

Synonyms: Follicle-stimulating hormone receptor, Follitropin receptor, FSH receptor, FSH-R, FSHRO, LGR1, ODG1, ovarian dysgenesis 1

Gene Symbol: FSHR

Molekulargewicht: 75 kDa

Gen-ID: 2492

UniProt: P23945

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Platelet-derived growth Factor Receptor Signaling