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FSHR Antikörper (N-Term)

FSHR Reaktivität: Human, Ratte, Affe, Rind (Kuh), Schwein, Fledermaus, Ziege, Pferd, Kaninchen, Schaf IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1048616
  • Target Alle FSHR Antikörper anzeigen
    FSHR (Follicle Stimulating Hormone Receptor (FSHR))
    Bindungsspezifität
    • 16
    • 16
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reaktivität
    Human, Ratte, Affe, Rind (Kuh), Schwein, Fledermaus, Ziege, Pferd, Kaninchen, Schaf
    Wirt
    • 63
    • 18
    Kaninchen
    Klonalität
    • 64
    • 17
    Polyklonal
    Konjugat
    • 34
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser FSHR Antikörper ist unkonjugiert
    Applikation
    • 58
    • 44
    • 26
    • 26
    • 20
    • 19
    • 13
    • 12
    • 6
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human FSHR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Rat, Sheep, Goat, Bat, Bovine, Rabbit, Horse, Pig (100%) Mouse, Elephant, Dog, Cat, Guinea pig, Sparrow (94%) Monkey, Hamster, Opossum (89%) Turkey (83%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 18 amino acid peptide from N-terminal extracellular domain of human FSHR. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Rat, Sheep, Goat, Bat, Bovine, Rabbit, Horse, Pig (100%), Mouse, Elephant, Dog, Cat, Guinea pig, Sparrow (94%), Monkey, Hamster, Opossum (89%), Turkey (83%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product FSHR Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (10 - 20 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    FSHR (Follicle Stimulating Hormone Receptor (FSHR))
    Andere Bezeichnung
    FSH Receptor / FSHR (FSHR Produkte)
    Synonyme
    FSHRO antikoerper, LGR1 antikoerper, ODG1 antikoerper, FSH-R antikoerper, eFSHR antikoerper, FSHRE antikoerper, fshr antikoerper, FSHR antikoerper, fshr-A antikoerper, GTHR-I antikoerper, zfGTHR-I antikoerper, LOC100304505 antikoerper, follicle stimulating hormone receptor antikoerper, follicle stimulating hormone receptor L homeolog antikoerper, follicle-stimulating hormone receptor antikoerper, FSHR antikoerper, fshr antikoerper, Fshr antikoerper, fshr.L antikoerper, Bm1_17785 antikoerper, LOC100304505 antikoerper
    Hintergrund
    Name/Gene ID: FSHR
    Subfamily: Glycoprotein hormone
    Family: GPCR

    Synonyms: FSHR, Follitropin receptor, FSHRO, Ovarian dysgenesis 1, FSH receptor, FSH-R, LGR1, ODG1
    Gen-ID
    2492
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Platelet-derived growth Factor Receptor Signaling
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