CDK2 Antikörper (CF®568)

Produktnummer ABIN7652820

Dieses Anti-CDK2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CDK2 in WB. Geeignet für Human, Maus und Xenopus laevis.

Kurzübersicht für CDK2 Antikörper (CF®568) (ABIN7652820)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reaktivität: Human, Maus, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDK2 Antikörper ist konjugiert mit CF®568

Applikation: Western Blotting (WB)

Klon: AN4-3

Produktdetails anti-CDK2 Antikörper (CF®568)

Verwendungszweck: Cdk2 / p34cdc2 Serine-Threonine Kinase(AN4.3), CF568 conjugate

Produktmerkmale: In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclindependent kinase 2 (Cdk2), also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2-related cyclin dependent kinases have been identified. These include Cdk3, Cdk4, Cdk5, PCTAIRE-1, PCTAIRE-2, PCTAIRE-3, Cdk6 Cdk7, Cdk8 and KKIALRE. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Cdk2 protein

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Inhibits activation of p34cdc2 kinase by cyclins, Immunoprecipitation, Kinase Assay, Western blot, Flow cytometry: 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Kommentare:

Positive Control: HeLa cells. HeLa whole cell lysate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Andere Bezeichnung: Cdk2

Hintergrund:

Synonyms: Cdc2 related protein kinase, CDC28, CDC2A, CDK1, CDK2, CDKN2, Cell devision kinase 2, Cell division protein kinase 2, cyclin dependent kinase 2 alpha, kinase Cdc2, MPF, p33 protein kinase

Gene Symbol: CDK2

Molekulargewicht: 34 kDa

Gen-ID: 1017

UniProt: P24941

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA