Cyclin A1 Antikörper (CF®647)

Produktnummer ABIN7652712

Dieses Anti-Cyclin A1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cyclin A1 in WB. Geeignet für Xenopus laevis.

Kurzübersicht für Cyclin A1 Antikörper (CF®647) (ABIN7652712)

Target: Cyclin A1 (CCNA1)

Reaktivität: Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin A1 Antikörper ist konjugiert mit CF®647

Applikation: Western Blotting (WB)

Klon: XLA1-3

Produktdetails anti-Cyclin A1 Antikörper (CF®647)

Verwendungszweck: Cyclin A1(XLA1-3), CF647 conjugate

Produktmerkmale: The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F, and retinoblastoma protein p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. The antibody detects Xenopus cyclin A in blots. Cyclin A1 is the embryonic cyclin which is present in Xenopus eggs. This MAb only detects Xenopus Cyclin A1. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length Xenopus laevis Cyclin A1 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Kommentare:

Positive Control: Very high levels in testis. Also found in myeloid leukemia cell lines. Xenopus laevis tissue lysate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Cyclin A1

Target: Cyclin A1 (CCNA1)

Andere Bezeichnung: Cyclin A1

Hintergrund:

Synonyms: CCN1, CCNA, CCNA2, Cyclin A1

Gene Symbol: ccna1

Molekulargewicht: 65 kDa

UniProt: P18606

Pathways: Apoptose, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA