ATG5 Antikörper (AA 1-119) (CF®488A)

Produktnummer ABIN7649706

Dieses Anti-ATG5-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ATG5 in WB, IHC und IHC (fp). Geeignet für Human.

Kurzübersicht für ATG5 Antikörper (AA 1-119) (CF®488A) (ABIN7649706)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATG5 Antikörper ist konjugiert mit CF®488A

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: ATG5-2101

Produktdetails anti-ATG5 Antikörper (CF®488A)

Bindungsspezifität: AA 1-119

Verwendungszweck: ATG5 (Autophagy Marker) (ATG5/2101), CF488A conjugate

Produktmerkmale: Autophagy protein 5 (ATG5), in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. ATG5 is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy, a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human ATG5 protein (around aa 1-119)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. ELISA: For coating use Ab at 1-5 μg/mL order Ab without BSA. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Western Blot 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: THP-1, PANC-1, Raji or HeLa cells. Brain, Ovary, Endometrium, Colon or Duodenum

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Andere Bezeichnung: ATG5

Hintergrund:

Synonyms: Autophagy protein 5, ATG5, Autophagy related 5, APG5-like, Apoptosis-specific protein

Gene Symbol: ATG5

Molekulargewicht: 50-32 kDa

Gen-ID: 9474

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagie