EGF Antikörper (Biotin)

Produktnummer ABIN7702924

Der Biotin-konjugierte Maus Monoklonal anti-EGF Antikörper (Klon C10) (ABIN7702924) detektiert spezifisch EGF in WB, IHC und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für EGF Antikörper (Biotin) (ABIN7702924)

Target: EGF (Epidermal Growth Factor (EGF))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EGF Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Klon: C10

Produktdetails anti-EGF Antikörper (Biotin)

Verwendungszweck: Biotin-Linked Monoclonal Antibody to Epidermal Growth Factor (EGF)

Spezifität: The antibody is a mouse monoclonal antibody raised against EGF. It has been selected for its ability to recognize EGF in immunohistochemical staining and western blotting.

Aufreinigung: Protein A + Protein G affinity chromatography

Immunogen: MAA560Ra21Monoclonal Antibody to Epidermal Growth Factor (EGF)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.5-2 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLOptimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: Epidermal Growth Factor

Hintergrund: URG, Beta-Urogastrone, Pro-epidermal growth factor

UniProt: P07522

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation