PRAS40 Antikörper

Produktnummer ABIN7606028

Dieses Anti-PRAS40-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von PRAS40 in WB und IP. Geeignet für Human, Maus und Ratte.

Kurzübersicht für PRAS40 Antikörper (ABIN7606028)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser PRAS40 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: ACHD-1

Produktdetails anti-PRAS40 Antikörper

Verwendungszweck: Anti-PRAS40 AKT1S1 Monoclonal Antibody

Produktmerkmale: Anti-PRAS40 AKT1S1 Monoclonal Antibody (ABIN7606028). Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PRAS40 Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt. One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 protein. PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000
IP 1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Konzentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Details zu PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Andere Bezeichnung: AKT1S1

Hintergrund:

Synonyms: Proline-rich AKT1 substrate 1,40 kDa proline-rich AKT substrate,AKT1S1 ,PRAS40 ,

Tissue Specificity: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293). .

Molekulargewicht: 58 kDa

UniProt: Q96B36

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Cell Size, Autophagie, BCR Signaling, Warburg Effekt