ZAP70 Antikörper (Middle Region)

Produktnummer ABIN7602981

Dieser Maus Monoklonal Antikörper erkennt spezifisch ZAP70 in WB und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für ZAP70 Antikörper (Middle Region) (ABIN7602981)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ZAP70 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Klon: 9D5

Produktdetails anti-ZAP70 Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: Anti-ZAP70 Antibody (monoclonal, 9D5)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-ZAP70 Antibody (monoclonal, 9D5) (ABIN7602981). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ZAP70, different from the related mouse sequence by two amino acids.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Andere Bezeichnung: ZAP70

Hintergrund:

Synonyms: SRK, STD, TZK, ZAP70, ZAP-70, ZAP 70, P43403

Tissue Specificity: Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.

Background: ZAP-70 (Zeta-chain-associated protein kinase 70) encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Molekulargewicht: 72 kDa

Gen-ID: 7535

UniProt: P43403

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway