GLI1 Antikörper (AA 800-1057)

Produktnummer ABIN7602517

Der Kaninchen Polyklonal Anti-GLI1-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, GLI1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für GLI1 Antikörper (AA 800-1057) (ABIN7602517)

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-GLI1 Antikörper

Bindungsspezifität: AA 800-1057

Verwendungszweck: Anti-GLI1 Antibody Picoband®

Produktmerkmale: Anti-GLI1 Antibody Picoband® (ABIN7602517). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human GLI1 recombinant protein (Position: Y800-Q1057). Human GLI1 shares 79.8% amino acid (aa) sequence identity with mouse GLI1.

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Kinzler KW, Bigner SH, Bigner DD, Trent JM, Law ML, O'Brien SJ, Wong AJ, Vogelstein B. (April 1987). "Identification of an amplified, highly expressed gene in a human glioma". Science 236 (4797): 70-3. 2. Ruiz i Altaba A. (June 1999). "Gli proteins encode context-dependent positive and negative functions: implications for development and disease.". Development 126 (14): 3205-16. 3. Sasaki H, Hui C, Nakafuku M, Kondoh H. (April 1997). "A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro". Development 124 (7): 1313-22.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu GLI1

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Andere Bezeichnung: GLI1

Hintergrund: GLI1, also known as GLI, is a protein originally isolated in human glioblastoma. The protein is encoded by GLI1 gene. It is mapped to 12q13.3. This gene is a member of a select group of cellular genes that are genetically altered in primary human tumors. GLI1 encodes a member of the GLI-Kruppel family of zinc finger proteins in the final steps of Hedgehog signaling in normal development and disease. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Beside, various upstream patterning signals may be integrated by the Gli proteins to a coherent programme of neurogenesis.

Molekulargewicht: 150 kDa

Gen-ID: 2735

UniProt: P08151

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis