KAP1 Antikörper (AA 699-835)

Produktnummer ABIN7602311

Dieses Anti-KAP1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von KAP1 in WB, IHC, IF, FACS, ICC und IHC (fro). Geeignet für Human, Maus und Ratte.

Kurzübersicht für KAP1 Antikörper (AA 699-835) (ABIN7602311)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 9E3

Produktdetails anti-KAP1 Antikörper

Bindungsspezifität: AA 699-835

Verwendungszweck: Anti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3) (ABIN7602311). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human KAP1 recombinant protein (Position: A699-P835). Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Capili AD, Schultz DC, RauscherIII FJ, Borden KL (Jan 2001). "Solution structure of the PHD domain from the KAP-1 corepressor: structural determinants for PHD, RING and LIM zinc-binding domains". The EMBO Journal 20 (1-2): 165-77. 2. Iyengar, Sushma, Farnham, Peggy (2011-07-29). "KAP1 Protein: An Enigmatic Master Regulator of the Genome". The Journal of Biological Chemistry 286. 3. Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A (May 2001). "The tripartite motif family identifies cell compartments". The EMBO Journal 20 (9): 2140-51.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Andere Bezeichnung: TRIM28

Hintergrund:

Synonyms: E3 SUMO protein ligase TRIM28 antibody, E3 SUMO-protein ligase TRIM28 antibody, FLJ29029 antibody, KAP 1 antibody, KAP-1 antibody, KRAB associated protein 1 antibody, KRAB interacting protein 1 antibody, KRAB-associated protein 1 antibody, KRAB-interacting protein 1 antibody, KRIP 1 antibody, KRIP-1 antibody, KRIP1 antibody, Nuclear corepressor KAP 1 antibody, Nuclear corepressor KAP-1 antibody, RING finger protein 96 antibody, RNF96 antibody, TF1B antibody, TIF1 beta antibody, TIF1-beta antibody, TIF1B antibody, TIF1B_HUMAN antibody, Transcription intermediary factor 1 beta antibody, Transcription intermediary factor 1-beta antibody, Trim28 antibody, Tripartite motif containing 28 antibody, tripartite motif containing protein 28 antibody, Tripartite motif-containing protein 28 antibody

Tissue Specificity: Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.

Background: Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1β (TIF1β) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.

Molekulargewicht: 100 kDa

Gen-ID: 10155

UniProt: Q13263

Pathways: Hedgehog Signalweg, Positive Regulation of Response to DNA Damage Stimulus