KAP1 Antikörper (AA 699-835)

Produktnummer ABIN7602310

Der Maus-Monoklonal Anti-KAP1-Antikörper wurde für WB, IHC, IF, FACS, ICC und IHC (fro) validiert. Er ist geeignet zum Nachweis von KAP1 in Proben aus Human, Maus und Ratte. }

Kurzübersicht für KAP1 Antikörper (AA 699-835) (ABIN7602310)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 3H2

Produktdetails anti-KAP1 Antikörper

Bindungsspezifität: AA 699-835

Verwendungszweck: Anti-KAP1/TRIM28 Antibody (monoclonal, 3H2)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-KAP1/TRIM28 Antibody (monoclonal, 3H2) (ABIN7602310). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human KAP1 recombinant protein (Position: A699-P835). Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Andere Bezeichnung: TRIM28

Hintergrund:

Synonyms: E3 SUMO protein ligase TRIM28 antibody, E3 SUMO-protein ligase TRIM28 antibody, FLJ29029 antibody, KAP 1 antibody, KAP-1 antibody, KRAB associated protein 1 antibody, KRAB interacting protein 1 antibody, KRAB-associated protein 1 antibody, KRAB-interacting protein 1 antibody, KRIP 1 antibody, KRIP-1 antibody, KRIP1 antibody, Nuclear corepressor KAP 1 antibody, Nuclear corepressor KAP-1 antibody, RING finger protein 96 antibody, RNF96 antibody, TF1B antibody, TIF1 beta antibody, TIF1-beta antibody, TIF1B antibody, TIF1B_HUMAN antibody, Transcription intermediary factor 1 beta antibody, Transcription intermediary factor 1-beta antibody, Trim28 antibody, Tripartite motif containing 28 antibody, tripartite motif containing protein 28 antibody, Tripartite motif-containing protein 28 antibody

Tissue Specificity: Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.

Background: Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1β (TIF1β) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.

Molekulargewicht: 100 kDa

Gen-ID: 10155

UniProt: Q13263

Pathways: Hedgehog Signalweg, Positive Regulation of Response to DNA Damage Stimulus