SKP1 Antikörper (AA 2-128)

Produktnummer ABIN7600465

Der Kaninchen Polyklonal Anti-SKP1-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, SKP1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für SKP1 Antikörper (AA 2-128) (ABIN7600465)

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SKP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-SKP1 Antikörper

Bindungsspezifität: AA 2-128

Verwendungszweck: Anti-SKP1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-SKP1 Antibody Picoband® (ABIN7600465). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human SKP1 recombinant protein (Position: P2-K128).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Arai, T., Kasper, J. S., Skaar, J. R., Ali, S. H., Takahashi, C., DeCaprio, J. A. Targeted disruption of p185/Cul7 gene results in abnormal vascular morphogenesis. Proc. Nat. Acad. Sci. 100: 9855-9860, 2003. 2. Bai, C., Sen, P., Hofmann, K., Ma, L., Goebl, M., Harper, J. W., Elledge, S. J. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell 86: 263-274, 1996. 3. Chen, H., Thalmann, I., Adams, J. C., Avraham, K. B., Copeland, N. G., Jenkins, N. A., Beier, D. R., Corey, D. P., Thalmann, R., Duyk, G. M. cDNA cloning, tissue distribution and chromosomal localization of Ocp2, a gene encoding a putative transcription-associated factor predominantly expressed in the auditory organs. Genomics 27: 389-398, 1995.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu SKP1

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Andere Bezeichnung: SKP1

Hintergrund:

Synonyms: S-phase kinase-associated protein 1, Cyclin-A/CDK2-associated protein p19, P19a, Organ of Corti protein 2, OCP-2, Organ of Corti protein II, OCP-II, RNA polymerase II elongation factor-like protein, SIII, Transcription elongation factor B polypeptide 1-like, p19skp1, SKP1, EMC19, OCP2, SKP1A, TCEB1L

Tissue Specificity: Ubiquitously expressed.

Background: Skp1 (S-phase kinase-associated protein 1) is a small protein of approximately 160 amino acids. It is mapped to 5q31.1. This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds ly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein (s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

Molekulargewicht: 19 kDa

Gen-ID: 6500

UniProt: P63208

Pathways: Zellzyklus, Hedgehog Signalweg, Mitotic G1-G1/S Phases