SPHK1 Antikörper (AA 16-267)

Produktnummer ABIN7600168

Dieses Anti-SPHK1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SPHK1 in WB, IHC, ELISA, FACS, IF und ICC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für SPHK1 Antikörper (AA 16-267) (ABIN7600168)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPHK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-SPHK1 Antikörper

Bindungsspezifität: AA 16-267

Verwendungszweck: Anti-SPHK1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-SPHK1 Antibody Picoband® (ABIN7600168). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human SPHK1 recombinant protein (Position: R16-H267).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kohama, T., Olivera, A., Edsall, L., Nagiec, M. M., Dickson, R., Spiegel, S. Molecular cloning and functional characterization of murine sphingosine kinase. J. Biol. Chem. 273: 23722-23728, 1998. 2. Melendez, A. J., Carlos-Dias, E., Gosink, M., Allen, J. M., Takacs, L. Human sphingosine kinase: molecular cloning, functional characterization and tissue distribution. Gene 251: 19-26, 2000. 3. Pitson, S. M., D'Andrea, R. J., Vandeleur, L., Moretti, P. A. B., Xia, P., Gamble, J. R., Vadas, M. A., Wattenberg, B. W. Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes. Biochem. J. 350: 429-441, 2000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Andere Bezeichnung: SPHK1

Hintergrund:

Synonyms: Hexokinase-2,2.7.1.1,Hexokinase type II,HK II,Muscle form hexokinase,HK2,

Tissue Specificity: Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.

Background: SPHK1(Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.

Molekulargewicht: 45-50 kDa

Gen-ID: 8877

Pathways: VEGF Signaling