TDG Antikörper (AA 15-370)

Produktnummer ABIN7600107

Der Kaninchen Polyklonal Anti-TDG Antikörper wurde für WB, ELISA, IF, ICC und FACS validiert. Er ist geeignet, TDG in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für TDG Antikörper (AA 15-370) (ABIN7600107)

Target: TDG (Thymine-DNA Glycosylase (TDG))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TDG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-TDG Antikörper

Bindungsspezifität: AA 15-370

Verwendungszweck: Anti-TDG Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-TDG Antibody (ABIN7600107). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human TDG recombinant protein (Position: Q15-R370).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu TDG

Target: TDG (Thymine-DNA Glycosylase (TDG))

Andere Bezeichnung: TDG

Hintergrund:

Synonyms: Kelch repeat and BTB domain-containing protein 2, BTB and kelch domain-containing protein 1, KBTBD2, BKLHD1, KIAA1489

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: G/T mismatch-specific thymine DNA glycosylase is an enzyme that in humans is encoded by the TDG gene. The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.

Molekulargewicht: 55-60 kDa

Gen-ID: 6996

UniProt: Q13569

Pathways: DNA Reparatur, Chromatin Binding