NSUN2 Antikörper (AA 146-654)

Produktnummer ABIN7600077

Der Kaninchen Polyklonal Anti-NSUN2-Antikörper wurde für WB, ELISA, IHC, IF, ICC und FACS validiert. Er ist geeignet, NSUN2 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für NSUN2 Antikörper (AA 146-654) (ABIN7600077)

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NSUN2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-NSUN2 Antikörper

Bindungsspezifität: AA 146-654

Verwendungszweck: Anti-NSUN2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NSUN2 Antibody Picoband® (ABIN7600077). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NSUN2 recombinant protein (Position: H146-K654).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Abbasi-Moheb, L., Mertel, S., Gonsior, M., Nouri-Vahid, L., Kahrizi, K., Cirak, S., Wieczorek, D., Motazacker, M. M., Esmaeeli-Nieh, S., Cremer, K., Weissmann, R., Tzschach, A., Garshasbi, M., Abedini, S. S., Najmabadi, H., Ropers, H. H., Sigrist, S. J., Kuss, A. W. Mutations in NSUN2 cause autosomal-recessive intellectual disability. Am. J. Hum. Genet. 90: 847-855, 2012. 2. Blanco, S., Bandiera, R., Popis, M., Hussain, S., Lombard, P., Aleksic, J., Sajini, A., Tanna, H., Cortes-Garrido, R., Gkatza, N., Dietmann, S., Frye, M. Stem cell function and stress response are controlled by protein synthesis. Nature 534: 335-340, 2016. 3. Blanco, S., Dietmann, S., Flores, J. V., Hussain, S., Kutter, C., Humphreys, P., Lukk, M., Lombard, P., Treps, L., Popis, M., Kellner, S., Holter, S. M., and 13 others. l Aberrant methylation of tRNAs links cellular stress to neuro-developmental disorders. EMBO J. 33: 2020-2039, 2014.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NSUN2

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Andere Bezeichnung: NSUN2

Hintergrund:

Synonyms: Transient receptor potential cation channel subfamily V member 3, TrpV3, Vanilloid receptor-like 3, VRL-3, TRPV3

Tissue Specificity: Abundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion. Expressed in the keratinocyte layers of the outer root sheath and, to lesser extent, to the matrix of the hair follicles.

Background: NOP2/Sun domain family, member 2 is a protein that in humans is encoded by the NSUN2 gene. This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Molekulargewicht: 100 kDa

Gen-ID: 54888

UniProt: Q08J23