CENPE Antikörper (AA 1390-2474)

Produktnummer ABIN7600005

Der Kaninchen Polyklonal Anti-CENPE-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, CENPE in Proben von Maus und Ratte zu detektieren.

Kurzübersicht für CENPE Antikörper (AA 1390-2474) (ABIN7600005)

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CENPE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-CENPE Antikörper

Bindungsspezifität: AA 1390-2474

Verwendungszweck: Anti-Cenpe Antibody Picoband®

Produktmerkmale: Anti-Cenpe Antibody Picoband® (ABIN7600005). Tested in WB, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Cenpe recombinant protein (Position: Q1390-Q2474). Mouse Cenpe shares 49.9% amino acid (aa) sequence identity with human Cenpe, respectively.

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Abrieu, A., Kahana, J. A., Wood, K. W., Cleveland, D. W. CENP-E as an essential component of the mitotic checkpoint in vitro. Cell 102: 817-826, 2000. 2. Barisic, M., Silva e Sousa, R., Tripathy, S. K., Magiera, M. M., Zaytsev, A. V., Pereira, A. L., Janke, C., Grishchuk, E. L., Maiato, H. Microtubule detyrosination guides chromosomes during mitosis. Science 348: 799-803, 2015. 3. Gross, M. B. Personal Communication. Baltimore, Md. 11/4/2014.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu CENPE

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Andere Bezeichnung: Cenpe

Hintergrund: Centromere-associated protein E is a protein that in humans is encoded by the CENPE gene. Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms.

Molekulargewicht: 300 kDa

Gen-ID: 229841

Pathways: M Phase, Maintenance of Protein Location