CDC25B Antikörper (AA 118-339)

Produktnummer ABIN7599793

Der Kaninchen Polyklonal anti-CDC25B Antikörper (ABIN7599793) detektiert spezifisch CDC25B in WB, ELISA und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Maus und Ratte.

Kurzübersicht für CDC25B Antikörper (AA 118-339) (ABIN7599793)

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDC25B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-CDC25B Antikörper

Bindungsspezifität: AA 118-339

Verwendungszweck: Anti-Cdc25b Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Cdc25b Antibody (ABIN7599793). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Cdc25b recombinant protein (Position: D118-D339).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Andere Bezeichnung: Cdc25b

Hintergrund:

Synonyms: C-C chemokine receptor type 1,C-C CKR-1,CC-CKR-1,CCR-1,CCR1,Macrophage inflammatory protein 1-alpha receptor,MIP-1alpha-R,RANTES-R,CD191,Ccr1,Cmkbr1,

Tissue Specificity: Detected in the heart, spleen, lung, peritoneal exudate cells and leukocytes.

Background: Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human CDC25 genes code for the A, B, and C forms of CDC25. CDC25B is mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and CDC25C at serine-216, phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.

Molekulargewicht: 65 kDa

Gen-ID: 12531

UniProt: P30306

Pathways: Zellzyklus, M Phase, Autophagie