GDNF Antikörper (Pro-Domain)

Produktnummer ABIN7581880

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GDNF in WB. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für GDNF Antikörper (Pro-Domain) (ABIN7581880)

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GDNF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-GDNF Antikörper

Bindungsspezifität: AA 54-66, Pro-Domain

Verwendungszweck: A Rabbit Polyclonal Antibody to proGDNF

Sequenz: (C)PEDYPDQFDD VMD

Kreuzreaktivität (Details): The antibody is specific for the pro-domain region of GDNF and will not react with mature GDNF.

Homologie: Rat,mouse - identical

Produktmerkmale: Anti-proGDNF Antibody (ABIN7581880) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize proGDNF from human, mouse, and rat samples. The antibody is specific for the pro-domain region of GDNF and will not react with mature GDNF.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)PEDYPDQFDDVMD, corresponding to amino acid residues 54-66 of human GDNF (precursor)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GDNF

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Andere Bezeichnung: GDNF

Hintergrund:

Synonyms: Glial cell line-derived neurotrophic factor precursor

Description: Glial-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily. GDNF signals through a multi-component receptor system, composed of a RET protooncogene and one of the four α1-α4 receptors1.GDNF promotes survival of various neuronal cells, including motoneurons2,3, Purkinje cells and sympathetic neurons4. In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake5. Cells that express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells6, neurons, pinealocytes, and skeletal muscle cells7.In vivo, following transection of facial motor neuron axons, locally applied GDNF has been shown to rescue virtually all damaged neurons from death8. GDNF may be of clinical relevance in the treatment of Parkinson's disease that is characterized by progressive degeneration of midbrain dopaminergic neurons9,10.Recently, it has been hypothesized that functional, carboxy-terminally amidated peptides are processed from the GDNF precursor upon proteolytic cleavage by furin-like endopeptidase11,12,13. Those different peptides (a 5-mer and 11-mer) have not been isolated endogenously to date. However, the rat 11-mer sequence (named brain excitatory peptide, BEP) significantly induced synaptic excitability and possessed some dopaminergic activities in vitro (thus named dopamine neuron stimulating peptides, DNSP)13. Furthermore, the human 11-mer sequence (named DNSP-11) exhibits neurotrophic-like properties13.Thus, the role of the full proDomain of GDNF, which is a product of proteolytic cleavage of proGDNF, is not clearly understood yet.

Gen-ID: 2668

UniProt: P39905

Pathways: RTK Signalweg, Synaptic Membrane, Tube Formation, Autophagie, Smooth Muscle Cell Migration