RFP Antikörper

Produktnummer ABIN7565988

Dieses Anti-RFP-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RFP in WB, ELISA, IF, IHC, FACS und FM. Geeignet für Discosoma.

Kurzübersicht für RFP Antikörper (ABIN7565988)

Target: RFP (Red Fluorescent Protein (RFP))

Reaktivität: Discosoma

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Produktdetails anti-RFP Antikörper

Verwendungszweck: RFP Antibody Pre-adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

Produktmerkmale: Synonyms: rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Aufreinigung:

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Preadsorption: Pre-Adsorbed

Sterilität: Sterile filtered

Immunogen:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.

Immunogen Type: Recombinant Protein

Anwendungsinformationen

Applikationshinweise: Application Note: RFP Antibody Pre-adsorbed is designed for detecting RFP and its variants, tested across ELISA, Western blot, IF, IHC, and suitable for EM, FC, FISH, IP, and multiplex assays. It is applicable for ELISA in both sandwich and capture formats. Researchers should determine optimal titers for specific applications. Recommended starting dilutions are: ELISA 1:28,700 - 1:48,700, FC, IF, IHC 1:200 - 1:2,000, WB 1:1,000 - 1:5,000. Flow Cytometry Dilution: 1:200 - 1:2,000 Immunohistochemistry Dilution: 1:200-1:2,000 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:20,000 - 1:50,000 IF Microscopy Dilution: 1:200-1:2,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.05 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu RFP

Target: RFP (Red Fluorescent Protein (RFP))

Andere Bezeichnung: DsRed

Hintergrund: Background: RFP Antibody Pre-adsorbed is a high-quality, rabbit polyclonal antibody specifically designed for the detection of red fluorescent protein (RFP) and its variants like mCherry, mScarlet, and tdTomato. This product is suitable for a broad range of applications including Western blot (WB), ELISA, immunohistochemistry (IHC), immunofluorescence (IF), flow cytometry (FC), and more. It is uniquely pre-adsorbed to minimize cross-reactivity, offering high specificity and sensitivity. With over 900 references, this product stands as a proven tool in the scientific community, supporting a wide range of research studies from cellular signaling to disease pathology.

UniProt: Q9U6Y8