IL1A Antikörper

Produktnummer ABIN7565966

Der Kaninchen Polyklonal Anti-IL1A-Antikörper wurde für WB, IHC und ELISA validiert. Er ist geeignet, IL1A in Proben von Human zu detektieren.

Kurzübersicht für IL1A Antikörper (ABIN7565966)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-IL1A Antikörper

Verwendungszweck: IL-1 Alpha Antibody

Kreuzreaktivität (Details): This antibody is primarily directed against the 17,000 MW human IL-1a and is useful in determining its presence in various assays.

Produktmerkmale: Synonyms: Interleukin 1 alpha precursor antibody, rabbit anti-Interleukin1 alpha antibody, rabbit anti-IL-1 Alpha Antibody, Pre-interleukin 1 alpha antibody

Aufreinigung: This is an IgG preparation of whole rabbit serum purified by DEAE fractionation.

Sterilität: Sterile filtered

Immunogen:

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli.  The MW of the recombinant IL-1a was 17,000.  This is the cleavage site generated by the IL-1b converting enzyme (ICE, capase-1).

Immunogen Type: Recombinant Protein

Anwendungsinformationen

Applikationshinweise: Application Note: This IgG fraction antibody of anti-Human IL-1a has been tested for use in immunoblotting. This antibody is suitable for use in ELISA, neutralizations, immunohistochemistry, and flow cytometry. It recognizes the 17,000 MW mature IL-1a.  Reactivity in other immunoassays is unknown. Immunohistochemistry Dilution: 1:100 to 1:200 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: IL1A

Hintergrund: Background: Interleukin 1 alpha is produced by activated macrophages. Anti-IL-1 alpha antibody is ideal for investigators involved in Immunology research.

Gen-ID: 3552

UniProt: P01583

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints