RFP Antikörper

Produktnummer ABIN7565910

Dieses Anti-RFP-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RFP in WB, ELISA, IHC, IP und FM. Geeignet für Discosoma.

Kurzübersicht für RFP Antikörper (ABIN7565910)

Target: RFP (Red Fluorescent Protein (RFP))

Reaktivität: Discosoma

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Produktdetails anti-RFP Antikörper

Verwendungszweck: Ready-To-Use RFP Antibody Pre-adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
 Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

Produktmerkmale: Synonyms: DsRed protein, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583, sea anemone Discosoma sp. Mushroom, RFP antibody, RFP-RTU, Ready To Use Antibody, RTU Antibody

Aufreinigung:

RTU Anti-RFP was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Preadsorption: Pre-Adsorbed

Sterilität: Sterile filtered

Immunogen:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.

Immunogen Type: Recombinant Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Ready-To-Use Anti-RFP is designed to detect RFP and its variants. Ready-To-Use Anti-RFP Rabbit Polyclonal Antibody has been optimized and tested in ELISA and in Western blot at a 1:1000 dilution. This Anti-RFP (RTU) Antibody is sufficient for 10 Western blots. Although not tested, there's a high likelihood that this antibody is functional in immunohistochemistry, immunofluorescence, and immunoprecipitation. Researchers can determine the best concentrations for these applications based on their specific experiments. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:1,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: User Optimized IF Microscopy Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.005 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.01 % Bovine Serum Albumin (BSA), 25 % (v/v) Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 2-8° C prior to opening. May aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to use.

Haltbarkeit: 12 months

Details zu RFP

Target: RFP (Red Fluorescent Protein (RFP))

Andere Bezeichnung: DsRed

Hintergrund: Background: Fluorescent proteins like green fluorescent protein (GFP) and Discosoma Red Fluorescent Protein (DsRFP) are widely used in research. DsRFP is derived from the mushroom anemone Discosoma sp. Despite sharing only 19 % similarity, anti-GFP antibodies typically don't recognize DsRFP and vice versa. Structurally, DsRFP is akin to GFP in its overall β-can fold and chromophore-formation chemistry. However, GFP undergoes an extra step in chromophore maturation and maintains a tetrameric structure. By using site-directed mutagenesis, various DsRFP variants have been engineered, enabling the red fluorescent protein to mature as a monomer. These monomeric DsRFP variants-mRFP1, mBanana, mCherry, mHoneydew, mPlum, mOrange, mStrawberry, and mTangerine-provide a diverse range of fluorescent colors. Rockland's RFP polyclonal antibodies, raised against the entire wild-type RFP protein, are expected to recognize all these variant forms. These antibodies have been pre-absorbed to prevent potential cross-reactivity with human, mouse, and rat serum proteins, and have also been confirmed to not react with GFP protein. All Rockland Immunochemical's RFP antibodies undergo stringent affinity purification to ensure high specificity and affinity. Thorough quality control tests guarantee that the final product meets or exceeds the highest standards, ensuring optimal performance in your assays.

UniProt: Q9U6Y8