RFP Antikörper

Produktnummer ABIN7565833

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch RFP in WB, ELISA und FM. Er zeigt eine Reaktivität gegenüber Discosoma.

Kurzübersicht für RFP Antikörper (ABIN7565833)

Target: RFP (Red Fluorescent Protein (RFP))

Reaktivität: Discosoma

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser RFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Produktdetails anti-RFP Antikörper

Verwendungszweck: RFP Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Red Fluorescent Protein (Discosoma).

Produktmerkmale: Synonyms: goat anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Aufreinigung: Anti-RFP (GOAT) is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Immunogen Type: Recombinant Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Polyclonal anti-RFP has been tested by and western blot and is designed to detect RFP and its variants. Optimal titers for applications should be determined by the researcher. Expect a band ~27 kDa in Western Blot in the appropriate cell lysate or extract. Western Blot Dilution: 1:1000-1:10,000 ELISA Dilution: 1:2,000 - 1:10,000 IF Microscopy Dilution: User Optimized Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu RFP

Target: RFP (Red Fluorescent Protein (RFP))

Andere Bezeichnung: DsRed

Hintergrund: Background: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS).

UniProt: Q9U6Y8