Leptin Antikörper

Produktnummer ABIN7565784

Dieses Anti-Leptin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Leptin in WB, IHC und ELISA. Geeignet für Maus.

Kurzübersicht für Leptin Antikörper (ABIN7565784)

Target: Leptin (LEP)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Leptin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-Leptin Antikörper

Verwendungszweck: Leptin Antibody

Kreuzreaktivität (Details): The antibody will recognize leptin from mouse and human.

Produktmerkmale: Synonyms: rabbit anti-leptin antibody, LEP antibody, Leptin Murine Obesity Homolog antibody, Leptin Precursor Obesity Factor antibody, OB antibody, Obese Protein antibody

Aufreinigung: Anti-Leptin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Leptin antibody was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.

Immunogen Type: Recombinant Protein

Anwendungsinformationen

Applikationshinweise: Application Note: This IgG fraction of anti-Mouse Leptin was tested for use in ELISA and immunoblotting.  Reactivity in other immunoassays is unknown. The antibody will recognize recombinant and native 16 kDa leptin from mouse and human. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Leptin

Target: Leptin (LEP)

Andere Bezeichnung: Lep

Hintergrund: Background: Anti-Leptin antibody detects leptin protein. Leptin may function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Obesity associated leptin is the product of the OB gene and has been identified with Type II diabetes. Anti-leptin Antibody is ideal for investigators involved in Cell Signaling, Obesity research, aging research and Signal Transduction research.

Gen-ID: 16846

UniProt: P41160

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process