Connexin 43/GJA1 Antikörper (Internal Region)

Produktnummer ABIN7540610

Hersteller Produkt- Nr.: 600-401-mj2s

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Connexin 43/GJA1 in WB, IHC, ELISA, IF und FM. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für Connexin 43/GJA1 Antikörper (Internal Region) (ABIN7540610)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Connexin 43/GJA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-Connexin 43/GJA1 Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 600-401-mj2s

Hersteller: Rockland

Verwendungszweck: Connexin 43 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against rat connexin 43.

Aufreinigung: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-Connexin 43 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse connexin 43 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000 - 1:15,000

Immunohistochemistry_Dilution: 1:200

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Connexin 43 Antibody has been tested in ELISA, WB, IF, and IHC. Expect a band at ~43Da in western blot using appropriate lysates. Positive control used: Mouse Heart in IHC, Rat Pup Brain cells, NIH-3T3, or HEK293T in IF, and HEK293T, Mouse Heart, or Rat Brain in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Andere Bezeichnung: Gja1

Hintergrund: Rabbit Anti-Connexin 43 Antibody, Rabbit Anti-Gja1 Antibody, Gap Junction Protein Alpha 1, Gap Junction Protein, Alpha 1, 43 kDa, Gap Junction 43 KDa Heart Protein, Connexin-43, GJAL, Gap Junction Protein, Alpha 1, 43 kDa (Connexin 43), Oculodentodigital Dysplasia (Syndactyly Type III), Gap Junction Protein, Alpha-Like, Gap Junction Alpha-1 Protein, Connexin 43, AVSD3, EKVP3, HLHS1, PPKCA, CMDR, CX43, EKVP, ODDD, Cx43, HSS,Connexin 43 or GJA1 (Gap Junction Protein Alpha 1) is a gap junction protein that acts as a regulator of bladder capacity. Gap channels (gap junctions) are specialized cell-cell contacts that provide direct intracellular communication. They allow passive diffusion of molecules up to 1 kDa, including nutrients, metabolites (glucose), ions (K+, Ca2+) and second messengers (IP3, cAMP). It may play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. Connexin 43 may play a role in cell growth inhibition through the regulation of NOV expression and localization. It plays an essential role in gap junction communication in the ventricles. Anti-Connexin 43 Antibody is useful for researchers interested in EGF/EGFR Signaling Pathways or signaling receptor binding.

Gen-ID: 14609

NCBI Accession: NP_034418

UniProt: P23242

Pathways: MAPK Signalweg, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization