Connexin 43/GJA1 Antikörper (Internal Region)

Produktnummer ABIN7540609

Hersteller Produkt- Nr.: 600-401-mj2

Dieses Anti-Connexin 43/GJA1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Connexin 43/GJA1 in WB, IHC, ELISA, IF und FM. Geeignet für Maus.

Kurzübersicht für Connexin 43/GJA1 Antikörper (Internal Region) (ABIN7540609)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Connexin 43/GJA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-Connexin 43/GJA1 Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 600-401-mj2

Hersteller: Rockland

Verwendungszweck: Connexin 43 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against rat connexin 43.

Aufreinigung: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-Connexin 43 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse connexin 43 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000 - 1:15,000

Immunohistochemistry_Dilution: 1:200

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Connexin 43 Antibody has been tested in ELISA, WB, IF, and IHC. Expect a band at ~43Da in western blot using appropriate lysates. Positive control used: Mouse Heart in IHC, Rat Pup Brain cells, NIH-3T3, or HEK293T in IF, and HEK293T, Mouse Heart, or Rat Brain in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Andere Bezeichnung: Gja1

Hintergrund: Rabbit Anti-Connexin 43 Antibody, Rabbit Anti-Gja1 Antibody, Gap Junction Protein Alpha 1, Gap Junction Protein, Alpha 1, 43 kDa, Gap Junction 43 KDa Heart Protein, Connexin-43, GJAL, Gap Junction Protein, Alpha 1, 43 kDa (Connexin 43), Oculodentodigital Dysplasia (Syndactyly Type III), Gap Junction Protein, Alpha-Like, Gap Junction Alpha-1 Protein, Connexin 43, AVSD3, EKVP3, HLHS1, PPKCA, CMDR, CX43, EKVP, ODDD, Cx43, HSS,Connexin 43 or GJA1 (Gap Junction Protein Alpha 1) is a gap junction protein that acts as a regulator of bladder capacity. Gap channels (gap junctions) are specialized cell-cell contacts that provide direct intracellular communication. They allow passive diffusion of molecules up to 1 kDa, including nutrients, metabolites (glucose), ions (K+, Ca2+) and second messengers (IP3, cAMP). It may play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. Connexin 43 may play a role in cell growth inhibition through the regulation of NOV expression and localization. It plays an essential role in gap junction communication in the ventricles. Anti-Connexin 43 Antibody is useful for researchers interested in EGF/EGFR Signaling Pathways or signaling receptor binding.

Gen-ID: 14609

NCBI Accession: NP_034418

UniProt: P23242

Pathways: MAPK Signalweg, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization