Calnexin Antikörper (C-Term)

Produktnummer ABIN7540514

Hersteller Produkt- Nr.: 200-401-e97

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Calnexin in WB, IHC, IF, IP und FACS. Er zeigt eine Reaktivität gegenüber Hund.

Kurzübersicht für Calnexin Antikörper (C-Term) (ABIN7540514)

Target: Calnexin (CANX)

Reaktivität: Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Calnexin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Produktdetails anti-Calnexin Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 200-401-e97

Hersteller: Rockland

Verwendungszweck: Calnexin-CT Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Calnexin -CT from Human, Monkey, Mouse, Rat, Bovine, Chicken (weak), Dog, Guinea pig, Hamster, Pig, Quail, Rabbit, Sheep, Drosophila (weak), and Xenopus (weak) based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Calnexin -CT Antibody was purified by Protein A chromatography.

Sterilität: Sterile filtered

Immunogen: Calnexin-CT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of dog calnexin. Identical to human, mouse and rat calnexin sequences over these residues.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: User Optimized

Western_Blot_Dilution: 1:2000

Kommentare:

Anti-Calnexin-CT Antibody is tested for use in WB, IP, IF microscopy, IHC and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Calnexin

Target: Calnexin (CANX)

Andere Bezeichnung: CANX

Hintergrund: CANX, CNX, IP90, P90, Calnexin, pp90,Calnexin, an abundant ~90 kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90. It consists of a large 50 kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail. Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention, calnexin has positively charged cytosolic residues that do the same thing. Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans. Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin.

Gen-ID: 403908

NCBI Accession: NP_001003232

UniProt: P24643

Pathways: MAPK Signalweg, Thyroid Hormone Synthesis