Calnexin Antikörper (N-Term)

Produktnummer ABIN6940953

Der Maus Monoklonal anti-Calnexin Antikörper wird verwendet zum Nachweis von Calnexin in Proben von Human. Er wurde validiert für WB, IHC und ELISA.

Kurzübersicht für Calnexin Antikörper (N-Term) (ABIN6940953)

Target: Calnexin (CANX)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Calnexin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Klon: CANX-1543

Produktdetails anti-Calnexin Antikörper

Bindungsspezifität: AA 1-300, N-Term

Verwendungszweck: Calnexin (Endoplasmic Reticulum Marker) Antibody

Spezifität: It recognizes a protein of 90 kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Calnexin

Target: Calnexin (CANX)

Andere Bezeichnung: CANX

Hintergrund:

Calnexin, CANX, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88, P90

HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Molekulargewicht: ~90kDa

Gen-ID: 821, 567968

UniProt: P27824

Pathways: MAPK Signalweg, Thyroid Hormone Synthesis