AMACR Antikörper

Produktnummer ABIN7538840

Der Maus Monoklonal Anti-AMACR-Antikörper wurde für IHC, IHC (p) und HIER validiert. Er ist geeignet, AMACR in Proben von Human zu detektieren.

Kurzübersicht für AMACR Antikörper (ABIN7538840)

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser AMACR Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Heat-induced Epitope Retrieval (HIER)

Klon: EPMU1

Produktdetails anti-AMACR Antikörper

Verwendungszweck: Liquid Mouse Monoclonal Antibody Alpha-Methylacyl-CoA Racemase (AMACR, p504S)

Spezifität: Human alpha-methylacyl-CoA racemase.

Produktmerkmale: This product is intended for the qualitative identification by light microscopy of alpha-methylacyl-CoA racemase (AMACR) molecules in paraffin sections.

Aufreinigung: Tissue culture supernatant

Immunogen: Prokaryotic recombinant protein corresponding to 382 amino acids of the human alpha-methylacyl-CoA racemase molecule.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on paraffin sections. Suggested dilution: 1:200 for 30 minutes at 25°C. Heat induced epitope retrieval using Epitope Retrieval Solution pH 9.0. This is provided as a guide and users should determine their own optimal working dilutions. Technical note: When utilised in immunohistochemistry, the use of H2O2 to block endogenous peroxidase has been shown to have a detrimental effect on the epitope recognized. It is, therefore, CRITICAL that blocking with H2O2 should be carried out after application of the primary antibody with solutions of no greater than 3 % . If these recommendations are not followed, staining intensity will be reduced. It is not recommended for use with PBS, since the use of PBS based wash buffers and possibly PBS based antibody diluents gives increased background staining and decreased staining intensity. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 84 mg/L

Buffer: This product is a liquid tissue culture supernatant containing 15 mM sodium azide as a preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Haltbarkeit: 12 months

Details zu AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Andere Bezeichnung: Alpha-Methylacyl-CoA Racemase

Pathways: Monocarboxylic Acid Catabolic Process