RAD50 Antikörper (AA 1-425)

Produktnummer ABIN7468849

Der Maus Monoklonal Anti-RAD50-Antikörper wurde für WB, IHC, IP, ICC, IF, IHC (p) und in vitro validiert. Er ist geeignet, RAD50 in Proben von Human zu detektieren.

Kurzübersicht für RAD50 Antikörper (AA 1-425) (ABIN7468849)

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAD50 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), In vitro Assay (in vitro)

Güteklasse: KO Validated

Klon: 13B3

Produktdetails anti-RAD50 Antikörper

Bindungsspezifität: AA 1-425

Kreuzreaktivität: Human, Affe, Maus, Ratte

Aufreinigung: Affinity purified by Protein G.

Immunogen: Amino acids 1-425 of Rad50 expressed in E. coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: HeLa , HeLa nuclear extract Validation: KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 2.24 mg/mL

Buffer: PBS, No Preservative

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu RAD50

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Andere Bezeichnung: RAD50 double strand break repair protein

Hintergrund:

Synonyms: RAD50 double strand break repair protein , NBSLD , RAD502 , hRad50

Background: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]

Molekulargewicht: 154 kDa

Gen-ID: 10111

UniProt: Q92878

Pathways: DNA Reparatur, Protein targeting to Nucleus