PCNA Antikörper (C-Term)

Produktnummer ABIN7455223

Der Kaninchen Polyklonal Anti-PCNA Antikörper wurde für WB, IP, IF (p) und IHC (fp) validiert. Er ist geeignet, PCNA in Proben von Human und Maus zu detektieren.

Kurzübersicht für PCNA Antikörper (C-Term) (ABIN7455223)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Produktdetails anti-PCNA Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Rabbit anti-PCNA Antibody, Affinity Purified

Homologie: Rat,Bovine

Aufreinigung: Affinity Purified

Immunogen: between AA 225 and C-term

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC-IF: 1:1,000 - 1:5,000

IHC: 1:2,000 to 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:5,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Hintergrund: Background: PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.

Gen-ID: 5111

NCBI Accession: NP_872590

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie