TDG Antikörper (AA 360-410)

Produktnummer ABIN7455026

Produktdetails anti-TDG Antikörper

Target: TDG (Thymine-DNA Glycosylase (TDG))

Bindungsspezifität: AA 360-410

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TDG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Verwendungszweck: Rabbit anti-TDG Antibody, Affinity Purified

Aufreinigung: Affinity Purified

Immunogen: Between AA 360 and 410

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu TDG

Target: TDG (Thymine-DNA Glycosylase (TDG))

Andere Bezeichnung: TDG (TDG Produkte)

Hintergrund: Background: Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine [taken from NCBI Entrez Gene (Gene ID: 6996)].

Gen-ID: 6996

NCBI Accession: NP_003202

UniProt: Q13569

Pathways: DNA Reparatur, Chromatin Binding