ZAP70 Antikörper (pTyr493, pTyr494)

Produktnummer ABIN7452236

Der Kaninchen Polyklonal Anti-ZAP70-Antikörper wurde für IP validiert. Er ist geeignet, ZAP70 in Proben von Human zu detektieren.

Kurzübersicht für ZAP70 Antikörper (pTyr493, pTyr494) (ABIN7452236)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZAP70 Antikörper ist unkonjugiert

Applikation: Immunoprecipitation (IP)

Produktdetails anti-ZAP70 Antikörper

Bindungsspezifität: pTyr493, pTyr494

Verwendungszweck: Rabbit anti-Phospho ZAP70 (Y493/Y494) Antibody, Affinity Purified

Homologie: Mouse

Aufreinigung: Affinity Purified

Immunogen: surrounding tyrosine 493/494

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IP: 2 - 5 μg/mg lysate

WB: Not recommended. ABIN7452236 has not performed satisfactorily when used for WB of phospho ZAP70 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ZAP70.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Andere Bezeichnung: ZAP70

Hintergrund: Background: ZAP-70 (zeta associated protein 70) is a tyrosine kinase of the Src family of tyrosine kinases. It was initially identified as a partner of the zeta chain of the CD3 complex associated to the T-cell receptor on T-lymphocytes. ZAP-70 shares homology with spleen-tyrosine kinase (Syk), a kinase involved in B-cell activation. Both ZAP-70 and Syk have been shown to be crucial to B- and T-lymphocyte development. In T-cells ZAP-70 functions to transduce the signaling cascade activated by T-cell receptor engagement and activation.

Gen-ID: 7535

NCBI Accession: NP_001070

UniProt: P43403

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway