Metabotropic Glutamate Receptor 5 Antikörper (C-Term)

Produktnummer ABIN7233043

Dieser Maus Monoklonal Antikörper detektiert spezifisch Metabotropic Glutamate Receptor 5 in WB, IHC, IP und IF/ICC. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für Metabotropic Glutamate Receptor 5 Antikörper (C-Term) (ABIN7233043)

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Metabotropic Glutamate Receptor 5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Klon: S75-3

Produktdetails anti-Metabotropic Glutamate Receptor 5 Antikörper

Bindungsspezifität: AA 824-1203, C-Term

Verwendungszweck: Anti-mGluR1/5 Glutamate Receptor Mouse Monoclonal Antibody

Spezifität: This antibody recognizes human, mouse, and rat mGluR1 and mGluR5.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein G affinity chromatography.

Immunogen: Fusion protein corresponding to aa 824-1203 (cytoplasmic C-terminus) of rat mGluR5b (accession no. P31424).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunoblotting: use at 1 μg/mL. A band of ~130 kDa is detected. Smaller fragments due to proteolysis are detected as well.
Positive control: Rat brain lysate.
These are recommended concentrations. User should determine optimal concentrations for their application.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Konzentration: Lot specific

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: This antibody is stable for at least one (1) year at -20°C.

Details zu Metabotropic Glutamate Receptor 5

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Andere Bezeichnung: Grm5

Hintergrund: Metabotropic glutamate receptor 5,G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium sPubMed:1320017, PubMed:21795692}.,The metabotropic glutamate receptors (mGluRs) are active through an indirect metabotropic process. They are members of the group C family of G-protein-coupled receptors, or GPCRs. Like all glutamate receptors, mGluRs bind glutamate which functions as an excitatory neurotransmitter. Metabotropic glutamate receptors are not ion channels. Instead, they activate biochemical cascades, leading to the modification of other proteins. This can lead to changes in the synapse's excitability. Eight different types of mGluRs, designated mGluR1 to mGluR8, are divided into groups I, II, and III. Receptor types are grouped based on receptor structure and physiological activity.,Cell membrane, Multi-pass membrane protein,mGluR5

UniProt: P31424

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity