Metabotropic Glutamate Receptor 5 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7886368

Der Kaninchen Polyklonal anti-Metabotropic Glutamate Receptor 5 Antikörper (ABIN7886368) detektiert spezifisch Metabotropic Glutamate Receptor 5 in WB, IHC, IF, FACS, IC und LCI. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für Metabotropic Glutamate Receptor 5 Antikörper (Extracellular, N-Term) (ABIN7886368)

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Metabotropic Glutamate Receptor 5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Produktdetails anti-Metabotropic Glutamate Receptor 5 Antikörper

Bindungsspezifität: Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 5

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, human - 13,14 amino residues identical

Produktmerkmale: Anti-mGluR5 (extracellular) Antibody (ABIN7043250, ABIN7044332 and ABIN7044333) is a highly specific antibody directed against an extracelluar epitope of rat mGluR5. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect live cell flow cytometry applications. It has been designed to recognize mGluR5 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:500

FC: 5 μg per reaction

ICC: 1:100

IHC: 1:50

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235697)

Blocking Peptide: (ABIN7235697)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Metabotropic Glutamate Receptor 5

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Andere Bezeichnung: GRM5

Hintergrund:

Synonyms: Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E

Description: L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptors family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus domain and an intracellular C-terminus one. The N-terminus domain of group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.mGluR1 and mGluR5 receptors signal through Gq/G11 that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. More downstream signaling pathways include activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins has also been reported.mGluR5 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the cortex, basal ganglia and hippocampus.The mGluR5 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinson's and Huntington's disease.

Gen-ID: 24418

NCBI Accession: NM_000842

UniProt: P31424

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity