ZAP70 Antikörper (pTyr315)

Produktnummer ABIN7224972

Der Kaninchen Polyklonal anti-ZAP70 Antikörper wird verwendet zum Nachweis von ZAP70 in Proben von Human und Maus. Er wurde validiert für WB, IHC, ELISA und IF.

Kurzübersicht für ZAP70 Antikörper (pTyr315) (ABIN7224972)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZAP70 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-ZAP70 Antikörper

Bindungsspezifität: pTyr315

Verwendungszweck: Rabbit Anti-ZAP-70 (phospho Tyr315) Polyclonal Antibody

Spezifität: Phospho-ZAP-70 (Y315) Polyclonal Antibody detects endogenous levels of ZAP-70 protein only when phosphorylated at Y315.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human ZAP-70 Phospho-Tyr315

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Andere Bezeichnung: ZAP-70

Hintergrund: ZAP70, SRK, Tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinaseZAP70 (zeta chain of T cell receptor associated protein kinase 70) encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in ZAP70 cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for ZAP70.

Molekulargewicht: 60kD

Gen-ID: 7535

UniProt: P43403

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway