RAD50 Antikörper (AA 650-730)

Produktnummer ABIN7216748

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch RAD50 in WB, IHC, ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für RAD50 Antikörper (AA 650-730) (ABIN7216748)

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD50 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-RAD50 Antikörper

Bindungsspezifität: AA 650-730

Verwendungszweck: Rabbit Anti-Rad50 Polyclonal Antibody

Spezifität: Rad50 Polyclonal Antibody detects endogenous levels of Rad50 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Rad50 at AA range: 650-730

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu RAD50

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Andere Bezeichnung: Rad50

Hintergrund: RAD50, DNA repair protein RAD50, hRAD50RAD50 double strand break repair protein encoded by RAD50 is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. RAD50 double strand break repair protein forms a complex with MRE11 and NBS1. RAD50 double strand break repair protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. RAD50 double strand break repair protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest RAD50 is essential for cell growth and viability. Mutations in RAD50 are the cause of Nijmegen breakage syndrome-like disorder.

Molekulargewicht: 153kD

Gen-ID: 10111

UniProt: Q92878

Pathways: DNA Reparatur, Protein targeting to Nucleus