PDK3 Antikörper (AA 170-250)

Produktnummer ABIN7230570

Der Kaninchen Polyklonal Anti-PDK3 Antikörper wurde für WB und ELISA validiert. Er ist geeignet, PDK3 in Proben von Human und Maus zu detektieren.

Kurzübersicht für PDK3 Antikörper (AA 170-250) (ABIN7230570)

Target: PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDK3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-PDK3 Antikörper

Bindungsspezifität: AA 170-250

Verwendungszweck: PDK3 Polyclonal Antibody

Spezifität: The antibody detects endogenous levels of PDK3 protein

Aufreinigung: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human PDK3 protein at AA range: 170-250

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu PDK3

Target: PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))

Andere Bezeichnung: PDK3

Hintergrund: Rabbit Anti-PDK3 Polyclonal Antibody,[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial , Pyruvate dehydrogenase kinase isoform 3,The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by PDK3 (Pyruvate Dehydrogenase Kinase 3) is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. PDK3 is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for PDK3.,PDK3

Molekulargewicht: observerd band 44kDa

Gen-ID: 5165

UniProt: Q15120

Pathways: PI3K-Akt Signalweg, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt