CD25 Antikörper (pSer268)

Produktnummer ABIN7221752

Der Kaninchen Polyklonal anti-CD25 Antikörper wird verwendet zum Nachweis von CD25 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, ELISA und IF.

Kurzübersicht für CD25 Antikörper (pSer268) (ABIN7221752)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-CD25 Antikörper

Bindungsspezifität: pSer268

Verwendungszweck: Rabbit Anti-IL-2Rα (phospho Ser268) Polyclonal Antibody

Spezifität: Phospho-IL-2Rα (S268) Polyclonal Antibody detects endogenous levels of IL-2Rα protein only when phosphorylated at S268.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human IL-2Ralpha Phospho-Ser268

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: IL-2Ralpha

Hintergrund: IL2RA, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD antigen CD25The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

Molekulargewicht: 55kD

Gen-ID: 3559

UniProt: P01589

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation