ELK1 Antikörper (pSer389)

Produktnummer ABIN7220224

Der Kaninchen Polyklonal anti-ELK1 Antikörper wird verwendet zum Nachweis von ELK1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, ELISA, IP und IF.

Kurzübersicht für ELK1 Antikörper (pSer389) (ABIN7220224)

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ELK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Produktdetails anti-ELK1 Antikörper

Bindungsspezifität: pSer389

Verwendungszweck: Rabbit Anti-Elk-1 (phospho Ser389) Polyclonal Antibody

Spezifität: Phospho-Elk-1 (S389) Polyclonal Antibody detects endogenous levels of Elk-1 protein only when phosphorylated at S389.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Elk-1 Phospho-Ser389

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IP 2-5 μg:mg lysate,ELISA 1:10000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu ELK1

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Andere Bezeichnung: Elk-1

Hintergrund: ELK1, ETS domain-containing protein Elk-1ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14.

Molekulargewicht: 48kD

Gen-ID: 2002

UniProt: P19419

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling