E2F1 Antikörper (pThr433)

Produktnummer ABIN7220110

Der Kaninchen Polyklonal Anti-E2F1 Antikörper wurde für WB und ELISA validiert. Er ist geeignet, E2F1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für E2F1 Antikörper (pThr433) (ABIN7220110)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-E2F1 Antikörper

Bindungsspezifität: pThr433

Verwendungszweck: E2F-1 (phospho Thr433) Polyclonal Antibody

Spezifität: Phospho-E2F-1 (T433) Polyclonal Antibody detects endogenous levels of E2F-1 protein only when phosphorylated at T433.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human E2F-1 Phospho-Thr433

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Andere Bezeichnung: E2F-1

Hintergrund: Rabbit Anti-E2F-1 (phospho Thr433) Polyclonal Antibody,E2F1, RBBP3, Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1,The E2F transcription factor 1 encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.,Transcription factor E2F1

Molekulargewicht: observerd band 70kDa

Gen-ID: 1869

UniProt: Q01094

Pathways: p53 Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagie