CDC25B Antikörper

Produktnummer ABIN7226172

Der Kaninchen Polyklonal anti-CDC25B Antikörper wird verwendet zum Nachweis von CDC25B in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA und IF.

Kurzübersicht für CDC25B Antikörper (ABIN7226172)

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDC25B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Produktdetails anti-CDC25B Antikörper

Verwendungszweck: Cdc25B Polyclonal Antibody

Spezifität: Cdc25B Polyclonal Antibody detects endogenous levels of Cdc25B protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cdc25B around the non-phosphorylation site of S323

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Andere Bezeichnung: Cdc25B

Hintergrund: Rabbit Anti-Cdc25B Polyclonal Antibody,CDC25B, CDC25HU2, M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B,CDC25B (cell division cycle 25B) is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.,M-phase inducer phosphatase 2

Molekulargewicht: observerd band 65kDa

Gen-ID: 994

UniProt: P30305

Pathways: Zellzyklus, M Phase, Autophagie