MAPK7 Antikörper (pSer731, pThr733)

Produktnummer ABIN710276

Der Kaninchen Polyklonal Anti-MAPK7-Antikörper wurde für WB, ELISA, IF (cc), IF (p), IHC (p) und IHC (fro) validiert. Er ist geeignet, MAPK7 in Proben von Human zu detektieren.

Kurzübersicht für MAPK7 Antikörper (pSer731, pThr733) (ABIN710276)

Target: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAPK7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-MAPK7 Antikörper

Bindungsspezifität: pSer731, pThr733

Spezifität: Recognizes Ser721+Thr723 in mice and rats

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Cow,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ERK5 around the phosphorylation sites of Ser731+Thr733

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu MAPK7

Target: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Andere Bezeichnung: ERK5+

Hintergrund:

Synonyms: BMK1, ERK4, ERK5, PRKM7, Mitogen-activated protein kinase 7, MAP kinase 7, MAPK 7, Big MAP kinase 1, BMK-1, Extracellular signal-regulated kinase 5, ERK-5, MAPK7

Background: Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.

Gen-ID: 5598

UniProt: Q13164

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, cAMP Metabolic Process, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling