Rekombinanter Keratin 77 Antikörper (AA 100-200)

Produktnummer ABIN7127261

Der Kaninchen Monoklonal anti-Keratin 77 Antikörper (Klon KRTL-4440R) (ABIN7127261) detektiert spezifisch Keratin 77 in IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter Keratin 77 Antikörper (AA 100-200) (ABIN7127261)

Target: Keratin 77 (KRT77)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Keratin 77 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: KRTL-4440R

Produktdetails anti-Keratin 77 Antikörper

Bindungsspezifität: AA 100-200

Verwendungszweck: Recombinant Cytokeratin, Type I (Epithelial Marker) Antibody

Spezifität: The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. It usually used in a cytokeratin cocktail with type II cytokeratin antibody.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Synthetic peptide corresponding to Type I Cytokeratins residues within aa100-200 of Type I Cytokeratins

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Skin or squamous cell carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Keratin 77

Target: Keratin 77 (KRT77)

Andere Bezeichnung: KRT77

Hintergrund:

K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B

Skin or squamous cell carcinoma.

Molekulargewicht: 60/53/51/45/40kDa

Gen-ID: 374454, 334989

UniProt: Q7Z794