Keratin 77 Antikörper

Produktnummer ABIN6941429

Der Maus Monoklonal anti-Keratin 77 Antikörper (Klon AE-1) (ABIN6941429) detektiert spezifisch Keratin 77 in IHC, WB, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Hund, Kaninchen, Rind (Kuh), Huhn, Affe und Schildkröte.

Kurzübersicht für Keratin 77 Antikörper (ABIN6941429)

Target: Keratin 77 (KRT77)

Reaktivität: Human, Ratte, Maus, Hund, Kaninchen, Rind (Kuh), Huhn, Affe, Schildkröte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 77 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: AE-1

Produktdetails anti-Keratin 77 Antikörper

Verwendungszweck: Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody

Spezifität: This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human epidermal keratin

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Skin or squamous cell carcinoma (IHC).

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Keratin 77

Target: Keratin 77 (KRT77)

Andere Bezeichnung: KRT77

Hintergrund:

K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Skin or squamous cell carcinoma (IHC).

Molekulargewicht: 40-56.5kDa

Gen-ID: 374454, 334989

UniProt: Q7Z794