Keratin 77 Antikörper

Produktnummer ABIN6937737

Der Maus Monoklonal anti-Keratin 77 Antikörper wird verwendet zum Nachweis von Keratin 77 in Proben von Human, Ratte, Maus, Hund, Kaninchen, Rind (Kuh), Huhn, Affe und Schildkröte. Er wurde validiert für IHC, WB, IF und FACS.

Kurzübersicht für Keratin 77 Antikörper (ABIN6937737)

Target: Keratin 77 (KRT77)

Reaktivität: Human, Ratte, Maus, Hund, Kaninchen, Rind (Kuh), Huhn, Affe, Schildkröte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 77 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: AE-1

Produktdetails anti-Keratin 77 Antikörper

Verwendungszweck: Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody

Spezifität: This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Kreuzreaktivität (Details): Human. Monkey. Cow. Dog. Rabbit. Mouse. Rat. Chicken. Turtle.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human epidermal keratin

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Skin or squamous cell carcinoma (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Keratin 77

Target: Keratin 77 (KRT77)

Andere Bezeichnung: KRT77

Hintergrund:

K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Skin or squamous cell carcinoma (IHC).

Molekulargewicht: 40-56.5kDa

Gen-ID: 374454, 334989

UniProt: Q7Z794