Keratin 77 Antikörper

Produktnummer ABIN6941447

Der Maus Monoklonal anti-Keratin 77 Antikörper wird verwendet zum Nachweis von Keratin 77 in Proben von Human und Ratte. Er wurde validiert für IHC.

Kurzübersicht für Keratin 77 Antikörper (ABIN6941447)

Target: Keratin 77 (KRT77)

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 77 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: KRTL-1077

Produktdetails anti-Keratin 77 Antikörper

Verwendungszweck: Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody

Spezifität: This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Kreuzreaktivität (Details): Shows broad species reactivity.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human KRT77 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Skin, Squamous cell carcinoma (SCC).

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Keratin 77

Target: Keratin 77 (KRT77)

Andere Bezeichnung: KRT77

Hintergrund:

K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B

Skin, Squamous cell carcinoma (SCC).

Molekulargewicht: 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)

Gen-ID: 374454, 334989

UniProt: Q7Z794