PCNA Antikörper

Produktnummer ABIN7126768

Dieses Anti-PCNA-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von PCNA in WB, IF, FACS und IHC (f). Geeignet für Ratte.

Kurzübersicht für PCNA Antikörper (ABIN7126768)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-PCNA Antikörper

Spezifität: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Kreuzreaktivität (Details): Human. Monkey. Pig. Mouse. Rat. Chicken. Zebrafish. Drosophila melanogaster. Yeast (S. pombe. S. cerevisiae). Dog

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Rat PCNA/protein A fusion protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A549, Tonsil or reactive lymph node.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Hintergrund: Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein,PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker)
Cellular localisation: Predominantly nuclear, some cytoplasmic

Molekulargewicht: 36kDa

Gen-ID: 5111, 147433, 728886

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie