PCNA Antikörper

Produktnummer ABIN7117811

Der Maus Monoklonal Anti-PCNA Antikörper wurde für WB, IHC, ELISA, FACS und IP validiert. Er ist geeignet, PCNA in Proben von Human, Maus, Ratte und Kaninchen zu detektieren.

Kurzübersicht für PCNA Antikörper (ABIN7117811)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human, Maus, Ratte, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Klon: 8F9

Produktdetails anti-PCNA Antikörper

Verwendungszweck: PCNA antibody

Aufreinigung: Protein A+G purification

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: proliferating cell nuclear antigen

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1:5000-1:50000, IP: 1:200-1:1000, IHC: 1:500-1:2000, IF: 1:50-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Hintergrund:

Synonyms: Proliferating cell nuclear antigen (PCNA)|Cyclin|PCNA

Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic(AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response(DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways(PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion(TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Molekulargewicht: 38 kDa

Gen-ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie