LIN28B Antikörper

Produktnummer ABIN7115882

Der Kaninchen Polyklonal Anti-LIN28B-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, LIN28B in Proben von Human und Maus zu detektieren.

Kurzübersicht für LIN28B Antikörper (ABIN7115882)

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIN28B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-LIN28B Antikörper

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: lin-28 homolog B(C. elegans)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Haltbarkeit: 12 months

Details zu LIN28B

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Andere Bezeichnung: LIN28B

Hintergrund: Synonyms:CSDD2 Background:Suppressor of microRNA(miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts(pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA(PubMed:22118463). Does not act on pri-miR21(PubMed:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation(PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent(PubMed:22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression(By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Molekulargewicht: 27 kDa

Gen-ID: 389421

UniProt: Q6ZN17